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Extra resources for CURRENT TOPICS IN DEVELOPMNTL BIOLOGY V6, Volume 6

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Soll e t al. (1969) have commented (p. 201) that “. . ” We call attention, therefore, to representative sections through encysted spores in which well defined gamma particles unquestionably exist (see Fig. , 1969). I n our experience, this situation is not a t all exceptional but, in fact, the rule. The MVB and numerous smaller vesicles are located in the vicinity of the gamma particles and near the cell wall. , 1969; Truesdell and Cantino, 1970) apparently in the process of fusing with the spore surface.

The absolute value of b is referred to as rate of inhibition because it is the rate a t which encystment decreases as population density increases; specifically, it is a measure of the decrease in R for every increase of lo6 spores/ml. Curves corresponding to Eq. (2) were also fitted to the data by regression techniques. R = a' + b' In p (2) The correlation coefficients for the linear and logarithmic equations were similar. This may mean that the true relationship between percent encystment and population density is not given by either relationship but, rather, that both relationships are almost equally good approximations.

B) Phase contrast view of a spore swollen by incubation a t O-l"C, showing nuclear cap, nucleus with nucleolus, swollen mitochondrion (arrow) with refractile lipid bodies (white dots) on surface and numerous gamma particles (small dark dots i n peripheral cytoplasm). ( C ) Same spore seen in (B), but after flagellum was absorbed while in the cold; note shift in position of nuclear apparatus toward center of cell. (D) Phase contrast view of an encysting spore decreasing in volume just after flagellar retraction ; note typical vacuoles.

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