By Renato Baserga, Christian Sell, Mitsuo Yoshinouchi (auth.), Melvin S. Oka Ph.D., Randall G. Rupp Ph.D. (eds.)
Cell Biology and Biotechnology: Novel ways to Increased mobile Productivity includes the court cases of the symposium by means of an analogous identify held in Cambridge, Massachusetts, January 30 - February 2, 1992. state of the art examine is gifted on: the IGF-1 Receptor and Gene Expression in the course of the cellphone Cycle; Attachment regulate of Fibroplast Proliferation; mobile Biology and Serum-Free Mouse Embryo Cells; Immunoglobulin construction Stimulating elements; Erythropoietin keep an eye on of Programmed loss of life in Erythroid Progenitors; Prohormone Processing Enzymes and Protein creation; regulate of Translation Initiation via Phosphorylation; Protein Retention within the Endoplasmic Reticulum Mediated via GPR78; Molecular methods in the direction of Manipulating the Expression of the Glucose similar Proteins in Mammalian Cells; Protein Folding within the Endoplasmic Reticulum; Sorting of Membrane Proteins within the Endocytic and Exocytic Pathways; CIS-Acting parts Which keep watch over Immunoglobulin Gene Transcription.
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Extra resources for Cell Biology and Biotechnology: Novel Approaches to Increased Cellular Productivity
9. Ruoslahti E. Fibronectin and its receptors. Annu Rev Biochem 1988;57: 375-413. 10. Vuorio E, de Crombrugghe B. The family of collagen genes. Annu Rev Biochem 1990;59:837-72. 11. Otsuka H, Moskowitz Ml. Arrest of 3T3 cells in Gl phase in suspension culture. 1 Cell Physiol 1975;87:213-20. 12. Matsuhisa T, Mori Y. An anchorage-dependent locus in the cell cycle for the growth of 3T3 cells. Exp Cell Res 1981;135:393-8. 13. Guadagno TM, Assoian RK. GlIS control of anchorage-independent growth in the fibroblast cell cycle.
This effect was not seen on 3T3 cells. SFME cells expressed another intermediate filament protein, vimentin, in the undifferentiated and differentiated state, consistent with the behavior of primary astrocytes in culture (18). The cells also underwent a striking morphological change within a few hours of exposure to TGF~, becoming more bipolar and adherent to the substratum (12). Markers for neurons, oligodendrocytes, and other cell types (galactocerebroside; 68, 160, and 200 kd neurofilaments; neuronal enolase; desmin; synaptophysin; and keratins) were not expressed, with or without TGF~ or serum treatment.
Once cells can grow at high density in the decreased concentration with or without the exogenous growth factors, the level of the autocrine factor can be kept high enough after it diffuses into the medium. Cells cultured at high density are more tolerant to such toxic metabolites as ammonium ion, peroxides, and lowered pH mainly caused by metabolically deposited lactic acid. Therefore, high-density culture not only cuts down the cost of the serum-free medium, but also assists the growth of cells that cannot proliferate in the same serum-free medium when inoculated at low density.