By M. L. Thakur (auth.), Max R. Hardeman PhD, Yves Najean MD (eds.)
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Additional resources for Blood cells in nuclear medicine, part I: Cell kinetics and bio-distribution
In 3 patients samples were analysed daily for up to 8 days following platelet labelling and in several other patients, samples were investigated on one or more occasions during this period. Using 750 WI volumes of blood pre- anG post-passage through the columns, the percentage radioactivity retained was calculated by direct measurement of radioactivity (Searle Automatic Gamma Systems). Following centrifugation of the same samples (Thrombofuge, Coulter Electronics Ltd) to produce platelet-rich plasma, a platelet count was made (Coulter Model FBI) and the percentage 47 of platelets retained calculated from the numerical data.
Half of them were studied with the gamma camera, the other half with the multicrystal external counting system. Among the 8 subjects, 6 received autologous non activated disc-shaped platelets and 2 autologous activated spherocytic platelets. In addition 3 asplenic patients (2 splenectomy, 1 functional asplenia) were studied. Results 1. Influence of the concentration of oxine on the aggregation of washed human platelets. Fig 1 shows that increasing the concentration of oxine, the carrier of 111 In through the platelet membrane, results in inhibition of platelet aggregation induced by ADP, collagen and thrombin.
Or chemical damage of the cells will, as has been stated before, also increase. Moreover, the active involvement of platelets in abscesses, using pure lllIn-oxinate labelled platelets, has been demonstrated (4). Finally, the suitability of mixed cell preparations, prepared according to this protocol, has been demonstrated recently for the clinical detection of inflammatory processes (5). Table 1. 35 ml sterile Tris-buffer is added to the lllIn-oxinate solution which is now ready for use. A quantity of the lipophylic oxinate complex, corresponding to the required dose of radioactivity (normally between 100-500 ~Ci) is transferred with the aid of a tuberculin syringe to the vial with the cell suspension and mixed carefully.