By Anne Houtman
Written by way of a technological know-how journalist and lecturers with over thirty years adventure within the lecture room, Biology Now skillfully blends middle biology ideas with renowned technology tales of genuine humans doing technology at the present time. those tales catch the human face of biology, highlighting the paintings of researchers and doctors who're making new discoveries each day. The textual content is followed through a wealth of conscientiously crafted pedagogy that teaches scholars the way to research technology within the information, interpret info, ask questions, and distinguish among technological know-how and pseudoscience.
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Additional info for Biology Now: Core Edition
1). On the first column synthesize the onginal peptide, on the other columns substitute each position of the peptide by all 20 amino acids m alphabetical order. Carry out the peptide synthesis steps as described in the previous sections. Here are some recommendations for the pipeting order. 9. Synthesis of a Pepticfe Scanning Library The first step is the derivation of the peptrde sequences from the protein sequence. A peptide length of 12 amino acids and an overlap of 9 amino acids is recommended In Fig.
11. ) have to be optimized for each system. In general, if you do not get any srgnal or your signals are too weak there are several posstbilities to optimize: a. Increase the ligand and/or antibody concentrations. b Prolong the incubation time with ligand to overnight at 4°C. c. Prolong the mcubation time with secondary antibody to 3 h. d. Shorten the washing times, use washing buffer without Tween-20. e. Shorten the blockmg time to 3 h. If the background IS too high, try the followmg changes: a.
38 Kramer and Schneider-Mergener Acknowledgments This work was supported by the BMBF, DFG, and Fonds der Chemlschen Industrie. We thank Beret Hoffmann and Christlane Landgraf for excellent technical assistance. References 1 Frank, R (1992) Spot synthesis. an easy technique for the positionally addressable, parallel chemical synthesis on a membrane support Tetrahedron 48, 9217-9232. 2. , Volkmer-Engert, R , Maim, R , Remeke, U , and SchneiderMergener, J (1993) Simultaneous synthesis of peptide libraries on single resin and contmuous membrane supports: identification of protem, metal and DNA bmdmg peptide mixtures.