By Holst, Otto
Skilled researchers describe in step by step aspect robust equipment for the research of bacterial exo- and endotoxins. those state of the art thoughts variety from purification and detection equipment to tools of conformational research, and contain using phage antibody libraries and strategies of structural and sensible analyses of endotoxins. each one protocol is defined via a scientist who has frequently used the strategy and optimized it to a excessive point of functionality. large notes take care of the problems which can come up while utilizing the tactic and with the method's barriers and attainable amendment for different reasons. state of the art and geared to generating profitable experimental effects, Bacterial pollutants: tools and Protocols offers investigators with a chief number of with no trouble reproducible tools designed to assist ultra-modern bacterial toxin investigators-both amateur and expert-utilize a big selection of strong learn instruments.
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Additional info for Bacterial Toxins: Methods and Protocols (Methods in Molecular Biology Vol 145)
Crit. Rev. Microbiol. 18, 115–158. 21. Welch, R. A. (1991) Pore-forming cytolysins of Gram-negative bacteria. Mol. Microbiol. 5, 521–528. 20 Alouf 22. Coote, J. (1992) Structural and functional relationships among the RTX toxin determinants of Gram-negative bacteria. FEMS Microbiol. Rev. 88, 137–162. 23. Titball, R. W. (1999) Membrane-damaging and cytotoxic phospholipases, in Bacterial Protein Toxins: A Sourcebook (Alouf, J. E. and Freer, J. ) Academic Press, London, England, pp. 310–329. 24. Alouf, J.
9 mg/mL of the complex under these running conditions. 12. 63 for type A neurotoxin. Analysis of pure neurotoxin off of the second DEAESephadex column by SDS-PAGE should show one band (Mr = 150 kDa) when unreduced (Fig. 1). Toxin should be >98% homogeneous and have a specific toxicity of >108LD50/mg. 38 Malizio, Goodnough, and Johnson 13. If the needle is in the vein, the plunger of the syringe will slide easily. A drop of blood will appear immediately upon retraction of the needle. If the needle is not in the vein, the plunger will be difficult to depress.
62. , and Aktories, K. (1997) Gln 63 of Rho is deamidated by Escherichia coli cytotoxic necrotizing factor-1. Nature 387, 725–729. 63. , and Aktories, K. (1996) Clostridium novyi α-toxin-catalyzed incorporation of GlcNac into Rho subfamily proteins. J. Biol. Chem. 271, 25,173–25,177. 64. , Murphy, J. , Lukianov, E. , and Boquet, P. (1997) Membrane translocation of diphtheria toxin fragment A exploits early to late endosome trafficking machinery. Mol. Microbiol. 23, 445–457. 65. Cover, T. L. and Blaser, M.