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By Harry ed. Sobotka

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The ratio between the rates of travel of the solvent and the particular compound in question is constant under standard conditions and is designated the Rf of that compound: distance traveled by compound Rf = distance traveled by solvent It is, however, constant only under specified standard conditions, and in practice comparison with authentic standard substances is necessary. 2. Paper Chromatography of Sugars Paper chromatography was first applied to the separation of sugars by Partridge ( P l ) .

H1. Herschel, Sir J. , Treatises of sound and light. , p. 438. J. J. Griffin, London, 1848. 28 I. MACINTYRE J1. -Sur l’analyse spectrale quantitative. Compt. rend. 71, 626-629 (1870). K1. , Chemische Analyse durch Spectralbeobachtungen. Ann. Physik Chern. 110, 181-189 ( 1860). L1. , “The Quantitative Spectral Analysis of the Elements,” Vol. 1. G. Fischer, Jena, 1929. L2. , “The Quantitative Spectral Analysis of the Elements,” Vol. 2. G. Fischer, Jena, 1934. , The flame-spectrophotometric determination of calcium in biological fluids and an isotopic analysis of the errors in the Kramer-Tisdall procedure.

76, 707-711 (1873). C2. Cholak, J,, and Hubbard, D. , Spectrochemical analysis with the airacetylene flame. Ind. Eng. , Anal. Ed. 16, 728-734 ( 1944). D1. Dean, J. ” McCraw-Hill, New York, 1960. D2. Domingo, W. , A photoelectric flame photometer. Biochem. J. 46,400408( 1949). E l . Ells, V. , The Lundegardh flame method of spectrographic analysis. J. Opt. SOC. Am. 31, 534-542 (1941). E2. Ells, V. , and Marshall, C. , The determination of exchangeable bases by the Lundegirdh spectrographic method.

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