By Oscar; Latner, A. L. Bodansky
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Both scales are logarithmic. mixture is carried round to a second dispensing position by the movement of the turntable, and the reagent is added which initiates the reaction. Stirring is effected by a rotating rod. The cuvette progresses into the light beam of the photometer. The absorbance reading is stored in a progranimable calculator, where it and subsequent readings on the same cuvette are used to compute catalytic concentration. The calculator, which forms part of the apparatus, is programmed by magnetic-tape cassette according to the type of analysis being performed.
A. Saunders, personal communication, 1975). These intervals could perhaps be reduced by the use of prewarmed reagents. Tiffany et al. (Tl) have shown that the within-run and between-run variabilities for serum transaminase assays carried out with a CentrifiChem analyzer are of the same order as those obtained with other ultraviolet-spectrophotonietric assays of these enzymes. 6% for elevated activities. 1%. Results obtained with the centrifugal analyzer were well correlated with those by other methods.
After a further interval (tr)the absorbances in the two reactions are compared simultaneously in a double-beam photometer. The difference in absorbance (AA) represents the progress of reaction in the time At. Limits are set on the choice of the interval between the successive reaction-initiation processes, since this determines the magnitude of AA, by the sensitivity of the photometer. W. MOSS Time FIG. 9. Determination of slope and linearity of the reaction progress curve in the Beckman System TR enzyme analyzer.