Download Advances in Botanical Research, Vol. 11 by J.A. Callow, H.W. Woolhouse (Eds.) PDF

By J.A. Callow, H.W. Woolhouse (Eds.)

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The instantaneous intensity is a C T c I - 10 n ( t )n 3 3 3 6 6 610 5 3 6 2 1 9 2 9 1 15 3 6 3 610 3 2 9 215 2 9 3 6 5 3 6 6 2 151 9 4 3 5 3 3 6 2 1 3 1 3 3 6 1 I 1 2 2 3 1 1 1 5 2 2101 3 L 5 2 i 6 z m z 3 4 3 1 1 0 1 6 2 6 4 2 6 2 5 1 9 1 5~. 3 3 1 2 1 2 3 6 5 2 2 3 70 n t 2 5 2 1 2 I . 3 2 1 1 1 1 2 10 2 3 2 6 1 1 I 2 1 2 7 . 1 1 2 2 I 3 I n 60 1 2 3 2 3 1 2 2 ~ 3215 1 3 6 3 1 9 1 3 3 3 1 2 1 3 2 3 1 3 6 3 2 3 4 6 1 6 1 6 4 2 3 2 6 1 6 1 6 2 2 3 1 2 8 4 6 n I 3 3 2 1 6 1 2 3 1 6 2 3 n n 40TIME (t)50 (t+ T ) 2 1 2 2 1 L 2 1 4 2 2 2 1 1 2 6 2 15 1 3 610 I 4 5 1 n 30 20 2 4 1 1 IS 2 5 3 5 1 1 3 1 3 1 1 1 4 LHI 2102 4 5 2 2 2101 I 2 3 1 1 6 2 3 1 -!

STEER ETAL. the increased signal levels found for the r2component from vesicle-rich areas mentioned earlier, this would mean that the local viscosities would have to be considerably higher, 75-750 CP for secretory vesicles and 25-500 CP for ribosomes (if these are assumed to be represented by the r2component). Such high viscosity conditions were encountered by Lehman and Pollard (1965) in extruded cytoplasm from bacterial cells, but they have not been reported from living cytoplasm. Even if these proposals are accepted, one question still remains unanswered: why does the overall cytoplasmic viscosity show so much variation?

The intensity of scattered light (I,)as a function of time (tin units of sample time) gives the instantaneous probability of detecting a photon. The number of photon detections within a sample time at time r is n ( t ) . At each time f , the individual products n(f)n(t+ T) are shown, the delay time T running vertically for each time t. These products are summed horizontally (over 50 sample times) to give the correlation function g ( 2 )(7)shown as X. Also plotted and 10,OM) samples (0). are correlation functions accumulated over loo0 samples (0) 24 M.

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